- It is the seventh theatrical Teenage Mutant Ninja Turtles film.
- Four years after the release of the 2016 live-action film Teenage Mutant Ninja Turtles: Out of the Shadows, an animated film was announced in June 2020 by future Paramount Pictures CEO Brian Robbins, with a new take putting the focus on the teenage aspect of the titular team.
- The film’s design was inspired by sketch drawings Rowe drew on his school notebooks as a teenager.
- This movie’s soundtrack was based on the video game Tony Hawk’s Pro Skater (1999).
- This is the first animated film to star both Jackie Chan and Seth Rogen since the Kung Fu Panda franchise.
- This is the second Teenage Mutant Ninja Turtles film where Splinter is Hamato Yoshi.
- This movie and its origin stories involved elements from both Mirage Comics and the first Teenage Mutant Ninja Turtles TV series.
- John Cena is the second wrestler to play Rocksteady, after Stephen Farrelly in Teenage Mutant Ninja Turtles: Out of the Shadows (2016).
- This is Seth Rogen’s 2nd time voicing a Warthog, after The Lion King (2019).
- This will be the second Teenage Mutant Ninja Turtles CGI movie that is released in theaters after TMNT (2007).
- The film and origin story uses elements from both the Mirage Comics and the 1987 cartoon.
- Jeff Rowe’s directorial debut.
- Teenage Mutant Ninja Turtles: Mutant Mayhem was released in the United States on August 2, 2023, by Paramount Pictures.